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Addgene

pFIN-RK-GFP-WPRE
(Plasmid #44358)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44358 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTY
  • Backbone manufacturer
    LJ Chang
  • Backbone size w/o insert (bp) 8760
  • Total vector size (bp) 9784
  • Modifications to backbone
    Final pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RK-GFP
  • Alt name
    human rhodopsin kinase promoter
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1024
  • Entrez Gene
    MAPK14 (a.k.a. CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA)
  • Promoter human rhodopsin kinase (pos 2557-2853)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The RK promoter was amplified from p150E1E2EG provided by Shahrokh C. Khani.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFIN-RK-GFP-WPRE was a gift from Susan Semple-Rowland (Addgene plasmid # 44358 ; http://n2t.net/addgene:44358 ; RRID:Addgene_44358)
  • For your References section:

    Expression characteristics of dual-promoter lentiviral vectors targeting retinal photoreceptors and Muller cells. Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, Pachigar K, Ludlow R, Khani SC, Smith WC. Mol Vis. 2010 May 27;16:916-34. PubMed 20517486