pFIN-RK-GFP-WPRE
(Plasmid
#44358)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44358 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTY
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Backbone manufacturerLJ Chang
- Backbone size w/o insert (bp) 8760
- Total vector size (bp) 9784
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Modifications to backboneFinal pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRK-GFP
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Alt namehuman rhodopsin kinase promoter
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1024
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Entrez GeneMAPK14 (a.k.a. CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA)
- Promoter human rhodopsin kinase (pos 2557-2853)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe RK promoter was amplified from p150E1E2EG provided by Shahrokh C. Khani.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFIN-RK-GFP-WPRE was a gift from Susan Semple-Rowland (Addgene plasmid # 44358 ; http://n2t.net/addgene:44358 ; RRID:Addgene_44358) -
For your References section:
Expression characteristics of dual-promoter lentiviral vectors targeting retinal photoreceptors and Muller cells. Semple-Rowland SL, Coggin WE, Geesey M, Eccles KS, Abraham L, Pachigar K, Ludlow R, Khani SC, Smith WC. Mol Vis. 2010 May 27;16:916-34. PubMed 20517486