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Addgene

pEIAV-SIN6.1 CBLacZ-LucWm1A
(Plasmid #44167)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44167 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega Corporation
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 7757
  • Vector type
    Lentiviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LacZ
  • Alt name
    beta-galactosidase
  • Species
    E. coli
  • Insert Size (bp)
    3100
  • GenBank ID
    NC_000913.2
  • Promoter CB, CMV enhancer/chicken beta-actin promoter

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    firefly luciferase
  • Species
    P. pyralis
  • Insert Size (bp)
    1650
  • Promoter CB, CMV enhancer/chicken beta-actin promoter

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEIAV-SIN6.1 CBLacZ-LucWm1A was a gift from John Olsen (Addgene plasmid # 44167 ; http://n2t.net/addgene:44167 ; RRID:Addgene_44167)
  • For your References section:

    High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. Patel M, Giddings AM, Sechelski J, Olsen JC. J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695. 10.1002/jgm.2695 PubMed 23319179