pEIAV-SIN6.1 CBLacZ-LucWm1A
(Plasmid
#44167)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44167 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI
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Backbone manufacturerPromega Corporation
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 7757
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Vector typeLentiviral, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameLacZ
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Alt namebeta-galactosidase
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SpeciesE. coli
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Insert Size (bp)3100
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GenBank IDNC_000913.2
- Promoter CB, CMV enhancer/chicken beta-actin promoter
Cloning Information for Gene/Insert 1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namefirefly luciferase
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SpeciesP. pyralis
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Insert Size (bp)1650
- Promoter CB, CMV enhancer/chicken beta-actin promoter
Cloning Information for Gene/Insert 2
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEIAV-SIN6.1 CBLacZ-LucWm1A was a gift from John Olsen (Addgene plasmid # 44167 ; http://n2t.net/addgene:44167 ; RRID:Addgene_44167) -
For your References section:
High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. Patel M, Giddings AM, Sechelski J, Olsen JC. J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695. 10.1002/jgm.2695 PubMed 23319179