pcDNA-p160(200-400)
(Plasmid
#44)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 myc-His A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep160 myb binding protein
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Alt nameMybbp1a
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Alt namep160MBP
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SpeciesM. musculus (mouse)
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Insert Size (bp)600
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MutationExpresses only aa200-400
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GenBank IDBC052889
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Entrez GeneMybbp1a (a.k.a. AL024407, AU019902, P160, p160MBP, p67MBP)
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Tag
/ Fusion Protein
- myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTom Gonda
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-p160(200-400) was a gift from Bruce Spiegelman (Addgene plasmid # 44 ; http://n2t.net/addgene:44 ; RRID:Addgene_44) -
For your References section:
Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK. Fan M, Rhee J, St-Pierre J, Handschin C, Puigserver P, Lin J, Jaeger S, Erdjument-Bromage H, Tempst P, Spiegelman BM. Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26. 10.1101/gad.1152204 PubMed 14744933