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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.2/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7711
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWNT9A
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SpeciesH. sapiens (human)
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Entrez GeneWNT9A (a.k.a. WNT14)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer TK-pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Active Wnt9A-V5 was a gift from Xi He (Addgene plasmid # 43820 ; http://n2t.net/addgene:43820 ; RRID:Addgene_43820) -
For your References section:
Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J Biol Chem. 2014 May 19. pii: jbc.M114.575027. 10.1074/jbc.M114.575027 PubMed 24841207