III-AGCT
(Plasmid
#43514)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3973
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Vector typeBacterial Cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIII-AGCT
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SpeciesSynthetic
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Insert Size (bp)426
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
III-AGCT was a gift from Chad Cowan & Kiran Musunuru (Addgene plasmid # 43514 ; http://n2t.net/addgene:43514 ; RRID:Addgene_43514) -
For your References section:
A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models. Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung RT, Musunuru K, Cowan CA. Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 10.1016/j.stem.2012.11.011. 10.1016/j.stem.2012.11.011 PubMed 23246482