pCTcon2
(Plasmid #41843)

• Depositing Lab: Dane Wittrup
• Publication: Chao et al Nat Protoc. 2006;1(2):755-68.

Backbone

• Vector backbone: pCT
• Backbone size w/o insert (bp): 6142
• Total vector size (bp): 6456
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CD20
• Insert Size (bp): 183
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • c-myc (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pCTcon2 genebank file
• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been found to be prone to multimerization. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.