pcDNA3-mNeptune2-N
(Plasmid #41645)

• Purpose: Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
• Depositing Lab: Michael Lin
• Publication: Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 6801
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeptune2-Dronpa145N
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1431
• Mutation: K145N on Dronpa (allows control of photochromic FP domain association by light)
• GenBank ID:
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: XpressFWD: TATGGCTAGCATGACTGGT
• 3′ sequencing primer: SP6: ATTTAGGTGACACTATAG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry