pMIEG3-NFAT2
(Plasmid
#40351)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMIEG3
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Backbone manufacturerDavid A. Williams (PMID: 10961859)
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFAT2
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Alt namenuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
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SpeciesM. musculus (mouse)
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GenBank IDNG_029226.1
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Entrez GeneNFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
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Tag
/ Fusion Protein
- IRES eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The NFAT2-expressing retroviruses were constructed by inserting blunt-ended NFAT2 cDNA from pSH160C into the retroviral vector pMIEG3 via the blunt-ended XhoI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIEG3-NFAT2 was a gift from Alexander Dent (Addgene plasmid # 40351 ; http://n2t.net/addgene:40351 ; RRID:Addgene_40351) -
For your References section:
Regulation of Th2 cytokine expression in NKT cells: unconventional use of Stat6, GATA-3, and NFAT2. Wang ZY, Kusam S, Munugalavadla V, Kapur R, Brutkiewicz RR, Dent AL. J Immunol. 2006 Jan 15;176(2):880-8. PubMed 16393972