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Addgene

pMIEG3-JunB
(Plasmid #40349)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40349 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMIEG3
  • Backbone manufacturer
    David A. Williams (PMID: 10961859)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JunB
  • Alt name
    Jun-B
  • Alt name
    jun B proto-oncogene
  • Species
    M. musculus (mouse)
  • GenBank ID
  • Entrez Gene
    Jun (a.k.a. AP-1, Junc, c-jun)
  • Tag / Fusion Protein
    • IRES eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The c-Jun- and JunB-expressing retroviruses were constructed by inserting c-Jun and JunB cDNAs into the retroviral vector pMIEG3 via NotI and XhoI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIEG3-JunB was a gift from Alexander Dent (Addgene plasmid # 40349 ; http://n2t.net/addgene:40349 ; RRID:Addgene_40349)
  • For your References section:

    Regulation of IL-10 gene expression in Th2 cells by Jun proteins. Wang ZY, Sato H, Kusam S, Sehra S, Toney LM, Dent AL. J Immunol. 2005 Feb 15;174(4):2098-105. PubMed 15699140