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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMIEG3
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Backbone manufacturerDavid A. Williams (PMID: 10961859)
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJunB
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Alt nameJun-B
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Alt namejun B proto-oncogene
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SpeciesM. musculus (mouse)
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GenBank ID
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Entrez GeneJun (a.k.a. AP-1, Junc, c-jun)
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Tag
/ Fusion Protein
- IRES eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The c-Jun- and JunB-expressing retroviruses were constructed by inserting c-Jun and JunB cDNAs into the retroviral vector pMIEG3 via NotI and XhoI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIEG3-JunB was a gift from Alexander Dent (Addgene plasmid # 40349 ; http://n2t.net/addgene:40349 ; RRID:Addgene_40349) -
For your References section:
Regulation of IL-10 gene expression in Th2 cells by Jun proteins. Wang ZY, Sato H, Kusam S, Sehra S, Toney LM, Dent AL. J Immunol. 2005 Feb 15;174(4):2098-105. PubMed 15699140