ERAP1
(Plasmid
#39174)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFB-CT10HF-LIC
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Backbone manufacturerSGC
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Vector typeBaculovirus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERAP1
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Alt nametype 1 tumor necrosis factor receptor shedding aminopeptidase regulator isoform a
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Alt namePDB:2XDT
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SpeciesH. sapiens (human)
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Insert Size (bp)2910
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Entrez GeneERAP1 (a.k.a. A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, PILS-AP, PILSAP)
- Promoter polyhedrin
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Tag
/ Fusion Protein
- TEV-His10-Flag (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Fbac-1
- 3′ sequencing primer Fbac-2 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ERAP1 was a gift from Nicola Burgess-Brown (Addgene plasmid # 39174 ; http://n2t.net/addgene:39174 ; RRID:Addgene_39174)