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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 38779 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnk1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)6381
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GenBank IDAF227741
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Entrez GeneWnk1 (a.k.a. Hsn2, Prkwnk1)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Low level of expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-myc-Wnk1 was a gift from Melanie Cobb (Addgene plasmid # 38779 ; http://n2t.net/addgene:38779 ; RRID:Addgene_38779) -
For your References section:
WNK1, a novel mammalian serine/threonine protein kinase lacking the catalytic lysine in subdomain II. Xu B, English JM, Wilsbacher JL, Stippec S, Goldsmith EJ, Cobb MH. J Biol Chem. 2000 Jun 2;275(22):16795-801. 10.1074/jbc.275.22.16795 PubMed 10828064