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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMXW-PGK-IRES-EGFP
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Backbone manufacturerChang-Zheng Chen Lab
- Backbone size (bp) 7066
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Vector typeMammalian Expression, Retroviral
- Promoter mPGK
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer mPGK-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MXW-PGK-IRES-EGFP was a gift from Chang-Zheng Chen (Addgene plasmid # 37648 ; http://n2t.net/addgene:37648 ; RRID:Addgene_37648) -
For your References section:
Loop nucleotides control primary and mature miRNA function in target recognition and repression. Yue SB, Trujillo RD, Tang Y, O'Gorman WE, Chen CZ. RNA Biol. 2011 Nov-Dec;8(6):1115-23. 10.4161/rna.8.6.17626 PubMed 22142974