UQ5827
(Bacterial strain #37174)

• Depositing Lab: Gary Roberts
• Publication: Gary Roberts unpublished plasmids (unpublished)

Backbone

• Vector backbone: pUX3056
• Modifications to backbone: ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: pRcoM1bs-R

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This strain contains Roberts lab plasmid pUX3056 which contains ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “d + e + f”
binding region.

For strain usage details see attached pdf. Please note that Addgene has not tested this plasmid functionally.