UQ5784
(Bacterial strain
#37173)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 37173 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonepUX3025
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Modifications to backbone~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This strain contains Roberts lab plasmid pUX3025 which contains ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “e + f”
binding region.
For strain usage details see attached pdf. Please note that this strain has not been tested functionally by Addgene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UQ5784 was a gift from Gary Roberts (Addgene plasmid # 37173)