UQ5876
(Bacterial strain
#37171)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 37171 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonepUX3009 AND pUX2410
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. Coli UQ5853
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Growth instructionsCopy number: pUX2410 High / pUX3009 Low
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecoxM1′::lacZ reporter system with RcoM binding sites "d + e + f" AND RcoM1 with C-term 6xHIS tag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pEXT20-F1
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3009 (coxM1′::lacZ reporter system with binding sites “d + e + f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3009 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UQ5876 was a gift from Gary Roberts (Addgene plasmid # 37171)