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Addgene

UQ5875
(Bacterial strain #37167)

Full plasmid sequence is not available for this item.

No maps are available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 37167 Bacteria in agar stab 1 $85

Backbone

  • Vector backbone
    pUX2996 and pUX2410

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. Coli UQ5853
  • Growth instructions
    Copy number: pUX2410 High / pUX2996 Low
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).

Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan.

For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UQ5875 was a gift from Gary Roberts (Addgene plasmid # 37167)