UQ5457
(Bacterial strain
#37165)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 37165 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backbonepUX2850
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. Coli VJS6737
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namercoM1 in pEXT20
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SpeciesBurkholderia xenovorans
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Tag
/ Fusion Protein
- 6xHIS (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pEXT20-F2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This strain contains Roberts lab plasmid pUX2850 which is an pUX2396 derivative suitable for expression of Burkholderia xenovorans RcoM1 protein + N-terminal 6×His tag. Host strain is E. coli VJS6737.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UQ5457 was a gift from Gary Roberts (Addgene plasmid # 37165)