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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMMP
- Backbone size (bp) 7037
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Modifications to backboneMCS (AgeI-HpaI-BstBI-NotI-XhoI-BamHI), IRES, eGFP
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Vector typeMammalian Expression, Retroviral
- Promoter CMV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sugden lab plasmid p3051
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMMP-MCS-IRES-eGFP was a gift from Bill Sugden (Addgene plasmid # 36953 ; http://n2t.net/addgene:36953 ; RRID:Addgene_36953) -
For your References section:
EBNA-1, a bifunctional transcriptional activator. Kennedy G, Sugden B. Mol Cell Biol. 2003 Oct;23(19):6901-8. 10.1128/MCB.23.19.6901-6908.2003 PubMed 12972608