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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMMP
- Backbone size (bp) 7037
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Modifications to backboneMCS (AgeI-HpaI-BstBI-NotI-XhoI-BamHI), IRES, Puro
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Vector typeMammalian Expression, Retroviral
- Promoter CMV
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN_5 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sugden lab plasmid p3048
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMMP-MCS-IRES-Puro was a gift from Bill Sugden (Addgene plasmid # 36952 ; http://n2t.net/addgene:36952 ; RRID:Addgene_36952) -
For your References section:
The LMP1 oncogene of EBV activates PERK and the unfolded protein response to drive its own synthesis. Lee DY, Sugden B. Blood. 2008 Feb 15;111(4):2280-9. Epub 2007 Nov 27. 10.1182/blood-2007-07-100032 PubMed 18042799
