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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBMP Receptor Type1A Q233D
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Alt nameBMPR1A
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SpeciesH. sapiens (human)
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Insert Size (bp)1602
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MutationGlutamine 233 to Aspartate
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Entrez GeneBMPR1A (a.k.a. 10q23del, ACVRLK3, ALK-3, ALK3, BMPR-1A, CD292, SKR5)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attL1
- 3′ sequencing primer attL2 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6-BMPR1A(Q233D)-V5 was a gift from Daniel Haber (Addgene plasmid # 35638 ; http://n2t.net/addgene:35638 ; RRID:Addgene_35638) -
For your References section:
TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA, Settleman J. Cell. 2012 Feb 17;148(4):639-50. 10.1016/j.cell.2011.12.033 PubMed 22341439
Map uploaded by the depositor.