pDEST14-SpyCatcher
(Plasmid #35044)

• Depositing Lab: Mark Howarth
• Publication: Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24.

Backbone

• Vector backbone: pDEST14
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyCatcher
• Alt name: CnaB2114
• Species: Streptococcus pyogenes
• Insert Size (bp): 420
• Mutation: Deletion of C-terminal till I113, and point mutations I34E and M69Y
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Supplemental Documents:
  • Note for Cloning of SpyTag
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry