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Addgene

pUAST_mLExA-VP16-NFATc1 (C-del)
(Plasmid #35023)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35023 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8900
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NFATc1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2669
  • Mutation
    deletion of amino acids 590-716
  • Entrez Gene
    NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
  • Promoter UAS
  • Tags / Fusion Proteins
    • mLexA (N terminal on insert)
    • VP16 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer LexA
  • 3′ sequencing primer hNFATc1_F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST_mLExA-VP16-NFATc1 (C-del) was a gift from Jing-W Wang (Addgene plasmid # 35023 ; http://n2t.net/addgene:35023 ; RRID:Addgene_35023)