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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAST
- Backbone size w/o insert (bp) 8900
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNFATc1
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SpeciesH. sapiens (human)
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Insert Size (bp)2669
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Mutationdeletion of amino acids 590-716
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Entrez GeneNFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
- Promoter UAS
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Tags
/ Fusion Proteins
- mLexA (N terminal on insert)
- VP16 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer LexA
- 3′ sequencing primer hNFATc1_F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST_mLExA-VP16-NFATc1 (C-del) was a gift from Jing-W Wang (Addgene plasmid # 35023 ; http://n2t.net/addgene:35023 ; RRID:Addgene_35023)