pBAD-PSmOrange2
(Plasmid #34957)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al J Am Chem Soc. 2012 Aug 17.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3963
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PSmOrange2
• Insert Size (bp): 747
• Mutation: R17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
• GenBank ID: JQ392581 NM_001018942

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: 5'-atgccatagcatttttatcc
• 3′ sequencing primer: 5' GAT TTA ATC TGT ATC AGG CTG

Resource Information

• Supplemental Documents:
  • PSmOrange2 alignment

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://www.ncbi.nlm.nih.gov/nuccore/JQ392581