Wnt10B Non-STOP
(Plasmid
#34748)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2580
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Vector typeGateway Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnt10B
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SpeciesH. sapiens (human)
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Insert Size (bp)1176
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MutationNone
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Entrez GeneWnt10b (a.k.a. Wnt1, Wnt12)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 (-20)-FWD
- 3′ sequencing primer M13-Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Wnt10B Non-STOP was a gift from Marian Waterman (Addgene plasmid # 34748 ; http://n2t.net/addgene:34748 ; RRID:Addgene_34748) -
For your References section:
A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. 10.1016/j.diff.2012.06.004 PubMed 22784633