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Purpose(Empty Backbone) Retroviral expression vector with pBabe compatible MCS
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV-IRES-RFP
- Backbone size (bp) 6575
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Modifications to backboneA pBabe compatible MCS was cloned between the original EcoRI and XhoI sites. The original EcoRI site was destroyed in the process, making it now BamHI-BstXI-SnaBI-EcoRI-BglII-XhoI
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note from the depositor: the EcoRI site is not unique as the site is also in the dsRed cDNA. In the mcs, the BamHI and XhoI sites are verified to be unique.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-pBabeMCS-IRES-RFP was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 33337 ; http://n2t.net/addgene:33337 ; RRID:Addgene_33337)
Map uploaded by Addgene staff.
