pMtBS
(Plasmid
#32226)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMONO-blasti
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Backbone manufacturerInvivoGen
- Backbone size w/o insert (bp) 3435
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Blasticidin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCV Small t antigen
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Alt nameMCPyV
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Alt nameMerkel Cell Polyomavirus
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Speciesvirus
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Insert Size (bp)560
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MutationSplice site was silently mutated, and a restriction site was silently added
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer TGAGCGGAGCTAATTCTCG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please consult laboratory website, http://home.ccr.cancer.gov/Lco/plasmids.asp, for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMtBS was a gift from Christopher Buck (Addgene plasmid # 32226 ; http://n2t.net/addgene:32226 ; RRID:Addgene_32226) -
For your References section:
Glycosaminoglycans and sialylated glycans sequentially facilitate merkel cell polyomavirus infectious entry. Schowalter RM, Pastrana DV, Buck CB. PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28. 10.1371/journal.ppat.1002161 PubMed 21829355