Skip to main content
Addgene

pCI-HA-ERK5-FL
(Plasmid #31817)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31817 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Erk5
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mapk7 (a.k.a. BMK-1, BMK1, ERK, ERK-5, ERK5, Erk5-, Erk5-T, PRKM7, b2b2346C, b2b2346Clo)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer EBV reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-HA-ERK5-FL was a gift from Astar Winoto (Addgene plasmid # 31817 ; http://n2t.net/addgene:31817 ; RRID:Addgene_31817)
  • For your References section:

    ERK5 is a novel type of mitogen-activated protein kinase containing a transcriptional activation domain. Kasler HG, Victoria J, Duramad O, Winoto A. Mol Cell Biol. 2000 Nov;20(22):8382-9. 10.1128/MCB.20.22.8382-8389.2000 PubMed 11046135