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Addgene

ACE2(-252)-luc
(Plasmid #31111)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31111 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    350
  • Mutation
    promoter region -252 to +103
  • GenBank ID
    NM_021804
  • Entrez Gene
    ACE2 (a.k.a. ACEH)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

promoter luciferase reporter

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACE2(-252)-luc was a gift from Gerhart Ryffel (Addgene plasmid # 31111 ; http://n2t.net/addgene:31111 ; RRID:Addgene_31111)
  • For your References section:

    Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU. Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90. 10.1016/j.bbaexp.2005.10.003 PubMed 16297991