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PurposeLuciferase reporter construct for human ACE2 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31111 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACE2 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)350
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Mutationpromoter region -252 to +103
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GenBank IDNM_021804
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Entrez GeneACE2 (a.k.a. ACEH)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ACE2(-252)-luc was a gift from Gerhart Ryffel (Addgene plasmid # 31111 ; http://n2t.net/addgene:31111 ; RRID:Addgene_31111) -
For your References section:
Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU. Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90. 10.1016/j.bbaexp.2005.10.003 PubMed 16297991