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Addgene

pSV-PGC1
(Plasmid #3)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 3 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV-Sport
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3160
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGC-1 alpha
  • Alt name
    PPAR gamma coactivator 1
  • Alt name
    PGC1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • GenBank ID
    NM_008904
  • Entrez Gene
    Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSV-PGC1 was a gift from Bruce Spiegelman (Addgene plasmid # 3 ; http://n2t.net/addgene:3 ; RRID:Addgene_3)

  • For your References section:

    A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Puigserver P, Wu Z, Park CW, Graves R, Wright M, Spiegelman BM. Cell 1998 Mar 20;92(6):829-39. 10.1016/S0092-8674(00)81410-5 PubMed 9529258
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