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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMyc-CMV
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3791
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebJunVN155(I152L)
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Alt namec-Jun(257-334)-Venus)1-154, I152L)
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SpeciesR. norvegicus (rat); and Aequorea Victoria
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Insert Size (bp)689
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MutationI152L in Venus(1-154)
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Entrez GeneJun
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To be published in Biotechniques. Please suggest users to cite the article later. Also, this plasmid can be used to replace pBiFC-bJunVN173.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBiFC-bJunVN155(I152L) was a gift from Chang-Deng Hu (Addgene plasmid # 27098 ; http://n2t.net/addgene:27098 ; RRID:Addgene_27098) -
For your References section:
An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio. Kodama Y, Hu CD. Biotechniques. 2010 Nov . 49(5):793-805. 10.2144/000113519 PubMed 21091444