pCS2+ tecktin-GFP
(Plasmid
#26932)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 26932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTektin 2
-
Alt nametekt2
-
SpeciesX. laevis (frog)
-
MutationI429A
-
Entrez Genetekt2
-
Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's sequencing results identified a I429A mutation in the Tektin 2 sequence when compared to GenBank reference sequence NP_001089680.1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS2+ tecktin-GFP was a gift from Christopher Kintner (Addgene plasmid # 26932 ; http://n2t.net/addgene:26932 ; RRID:Addgene_26932) -
For your References section:
Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells. Park TJ, Mitchell BJ, Abitua PB, Kintner C, Wallingford JB. Nat Genet. 2008 Jul . 40(7):871-9. 10.1038/ng.104 PubMed 18552847