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Addgene

pCS2+ tecktin-GFP
(Plasmid #26932)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26932 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tektin 2
  • Alt name
    tekt2
  • Species
    X. laevis (frog)
  • Mutation
    I429A
  • Entrez Gene
    tekt2
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results identified a I429A mutation in the Tektin 2 sequence when compared to GenBank reference sequence NP_001089680.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ tecktin-GFP was a gift from Christopher Kintner (Addgene plasmid # 26932 ; http://n2t.net/addgene:26932 ; RRID:Addgene_26932)
  • For your References section:

    Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells. Park TJ, Mitchell BJ, Abitua PB, Kintner C, Wallingford JB. Nat Genet. 2008 Jul . 40(7):871-9. 10.1038/ng.104 PubMed 18552847