mCherry-UtrCH
(Plasmid #26740)

• Depositing Lab: William Bement
• Publication: Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4097
• Vector type: Mammalian Expression ; reporter for F-actin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Utrophin
• Alt name: UtrCH
• Species: unknown
• Mutation: Calponin homology domain of utrophin (aa# 1-261)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • GBD/UtrCH pCS2+ author MCS map
• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The calponin homology domain of utrophin (Utr-CH) was fused to mCherry in order to visualize F-actin.

mCherry R154 mutated (CGG to AGA) for optimal codon usage in Xenopus.