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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameActin-binding Protein-1
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Alt namemAbp1
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SpeciesM. musculus (mouse)
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Entrez GeneDbnl (a.k.a. RP23-385C16.5, Abp1, SH3P7, mAbp1)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMouse mAbp1 was kindly provided by David Drubin (University of California-Berkeley)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPc2 mAbp1 was a gift from Anna Huttenlocher (Addgene plasmid # 26719 ; http://n2t.net/addgene:26719 ; RRID:Addgene_26719) -
For your References section:
Actin-binding protein-1 interacts with WASp-interacting protein to regulate growth factor-induced dorsal ruffle formation. Cortesio CL, Perrin BJ, Bennin DA, Huttenlocher A. Mol Biol Cell. 2010 Jan 1. 21(1):186-97. 10.1091/mbc.e09-02-0106 PubMed 19910490
Map uploaded by the depositor.