cp204
(Plasmid #25951)

• Depositing Lab: Robert Campbell
• Publication: Carlson et al Protein Sci. 2010 Jun 2. ():.

Backbone

• Vector backbone: pBad/HisB
• Backbone size w/o insert (bp): 4100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cp204
• Alt name: cp204
• Species: D. Striata
• Insert Size (bp): 800
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: RECA1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequence with pBAD-F shows minor mismatches and gaps with the reference sequence from the depositing lab. These discrepancies are a result of mistakes made during sequence assembly and should not impact plasmid function.

Please note that growing the construct at 30-34C may increase protein yield (36-48hours).