pCS2 xenopus DAN
(Plasmid
#24693)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDAN
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SpeciesX. laevis (frog)
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Entrez Genenbl1 (a.k.a. DAN, no3, dand1, nbl1-a)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense linearize with EcoRI, transcribe with T7
For sense linearize with NotI, transcribe with SP6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 xenopus DAN was a gift from Edward De Robertis (Addgene plasmid # 24693 ; http://n2t.net/addgene:24693 ; RRID:Addgene_24693) -
For your References section:
Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo. Pera EM, Hou S, Strate I, Wessely O, De Robertis EM. Int J Dev Biol. 2005 . 49(7):781-96. 10.1387/ijdb.052003ep PubMed 16172975