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Addgene

pBS xenopus paraxial protocadherin
(Plasmid #24687)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24687 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS-SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    xenopus paraxial protocadherin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3600
  • Entrez Gene
    pcdh8.2.L (a.k.a. XELAEV_18013228mg, arcadlin, papc, pcdh8.2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For antisense linearize with NotI
Transcribe with T7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS xenopus paraxial protocadherin was a gift from Edward De Robertis (Addgene plasmid # 24687 ; http://n2t.net/addgene:24687 ; RRID:Addgene_24687)
  • For your References section:

    The role of paraxial protocadherin in selective adhesion and cell movements of the mesoderm during Xenopus gastrulation. Kim SH, Yamamoto A, Bouwmeester T, Agius E, Robertis EM. Development. 1998 Dec . 125(23):4681-90. PubMed 9806917