pBS xenopus paraxial protocadherin
(Plasmid
#24687)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS-SK
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namexenopus paraxial protocadherin
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SpeciesX. laevis (frog)
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Insert Size (bp)3600
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Entrez Genepcdh8.2.L (a.k.a. XELAEV_18013228mg, arcadlin, papc, pcdh8.2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense linearize with NotI
Transcribe with T7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS xenopus paraxial protocadherin was a gift from Edward De Robertis (Addgene plasmid # 24687 ; http://n2t.net/addgene:24687 ; RRID:Addgene_24687) -
For your References section:
The role of paraxial protocadherin in selective adhesion and cell movements of the mesoderm during Xenopus gastrulation. Kim SH, Yamamoto A, Bouwmeester T, Agius E, Robertis EM. Development. 1998 Dec . 125(23):4681-90. PubMed 9806917
Map uploaded by the depositor.