pcDNA3 xenopus twisted gastrulation
(Plasmid
#24680)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24680 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametwisted gastrulation
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SpeciesX. laevis (frog)
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Insert Size (bp)970
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Entrez Genetwsg1 (a.k.a. tsg, twsg, twsg1-b, MGC69051)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bstx1 (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Best for in situs
For sense injection:
Cut with BamHI
Transcribe with SP6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 xenopus twisted gastrulation was a gift from Edward De Robertis (Addgene plasmid # 24680 ; http://n2t.net/addgene:24680 ; RRID:Addgene_24680) -
For your References section:
The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling. Oelgeschlager M, Larrain J, Geissert D, De Robertis EM. Nature. 2000 Jun 15. 405(6788):757-63. 10.1038/35015500 PubMed 10866189