pcDNA3-PKCZeta KR HA Tagged
(Plasmid
#24608)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24608 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKCZeta
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Alt namePKC zeta
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SpeciesR. norvegicus (rat)
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MutationK281R mutation in the ATP binding domain causing a kinase dead variant.
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GenBank IDNM_022507.1 NP_071952.1
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Entrez GenePrkcz (a.k.a. 14-3-3-zetaisoform, Pkcz, r14-3-3)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-PKCZeta KR HA Tagged was a gift from Jeff Wrana (Addgene plasmid # 24608 ; http://n2t.net/addgene:24608 ; RRID:Addgene_24608) -
For your References section:
Regulation of cell polarity and protrusion formation by targeting RhoA for degradation. Wang HR, Zhang Y, Ozdamar B, Ogunjimi AA, Alexandrova E, Thomsen GH, Wrana JL. Science. 2003 Dec 5. 302(5651):1775-9. 10.1126/science.1090772 PubMed 14657501