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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAC-GH146
- Backbone size w/o insert (bp) 18000
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQF
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SpeciesNeuropspora crassa
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Insert Size (bp)2450
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Tag
/ Fusion Protein
- dsRed (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AvrI (not destroyed)
- 5′ sequencing primer DsRed1-C (Common Sequencing Primers)
Resource Information
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Reference
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBAC-3xPDsRed-GH146-MCS with FseI-AscI-AvrII multi-cloning site has been
previously described (Hong et al., 2009). QF cDNA with 5’ FseI and 3’ Avr restriction sites was
amplified from pAC-QF by PCR, and cloned into the FseI/AvrII restriction sites of pBAC-GH146 to
yield pBac-GH146-QF.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBac-GH146-QF was a gift from Liqun Luo (Addgene plasmid # 24353 ; http://n2t.net/addgene:24353 ; RRID:Addgene_24353) -
For your References section:
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990