pmCD8-GFP,y+
(Plasmid #24350)

• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Backbone

• Vector backbone: pGalW
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Yellow gene
• Species: D. melanogaster (fly)
• Insert Size (bp): 9000
• Tag / Fusion Protein:
  • CD8-GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: GFP-F

Resource Information

• Reference:
  • pmCD8-GFP,y+ (GenBank)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This enhancer trap vector is based on the pGalW vector (Gerlitz et al., 2002). The
GAL4 insert from pGalW was removed by complete NotI and partial XbaI digestion and replaced
with the GAL80 ORF isolated as a NotI/XbaI fragment from pCasper-tubP-GAL80 (Lee and Luo,
1999), to generate the plasmid pG80,w+. To generate pG80,y+, the white gene from pG80,w+ was
excised by EcoRV/SacII digestion, the vector was blunted with Klenow, and replaced with the
yellow gene as a blunted SalI fragment from the y S/G plasmid (a gift from Pamela Geyer,
University of Iowa). To generate pmCD8-GFP,y+, the GAL80 insert was excised by NotI digestion
and replaced with mCD8-GFP which was PCR amplified from pUAST-mCD8GFP to include 5' and
3' NotI restriction sites (see also Berdnik et al., 2006).