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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK(+)
- Backbone size (bp) 3000
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Modifications to backboneGFP split with an intron (GG)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 5XGAL4 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer GFP-N (Common Sequencing Primers)
Resource Information
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Reference
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The BamHI/BglI fragment from pUAST (Brand and Perrimon, 1993), containing 5 copies
of a GAL4 binding site and the Drosophila hsp70 minimal promoter was subcloned into BamHI site
of pBluescript containing an optimized GFP split with an intron (GG) (Zong et al., 2005). This
strategy generates a hybrid BglII/BamHI site in front of the GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAS-GG was a gift from Liqun Luo (Addgene plasmid # 24342 ; http://n2t.net/addgene:24342 ; RRID:Addgene_24342) -
For your References section:
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990