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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFPC1
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFATc1
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Alt nameNFAT2
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SpeciesH. sapiens (human)
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Insert Size (bp)2147
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Entrez GeneNFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- EE (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer EGFP C seq. primer (#6478-1) (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFPC1-huNFATc1EE-WT was a gift from Jerry Crabtree (Addgene plasmid # 24219 ; http://n2t.net/addgene:24219 ; RRID:Addgene_24219) -
For your References section:
Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Beals CR, Clipstone NA, Ho SN, Crabtree GR. Genes Dev. 1997 Apr 1. 11(7):824-34. 10.1101/gad.11.7.824 PubMed 9106655