pGL3-p27 T187A Luc
(Plasmid
#23048)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3-control
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5223
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep27
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Alt namekip1
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SpeciesH. sapiens (human)
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Insert Size (bp)607
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MutationT187A: mutation at codon 187 changing from T (ACG) to A (GCG), additionally 3 silent mutations introducing a BamH I site at codons 182/183/184 (GGT TCT GTG) to (GGA TCC GTC)
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GenBank IDU10906
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Entrez GeneCDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
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Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Nco I (not destroyed)
- 5′ sequencing primer GLprimer2 (Promega) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-p27 T187A Luc was a gift from William Kaelin (Addgene plasmid # 23048 ; http://n2t.net/addgene:23048 ; RRID:Addgene_23048) -
For your References section:
Bioluminescent imaging of Cdk2 inhibition in vivo. Zhang GJ, Safran M, Wei W, Sorensen E, Lassota P, Zhelev N, Neuberg DS, Shapiro G, Kaelin WG. Nat Med. 2004 Jun . 10(6):643-8. 10.1038/nm1047 PubMed 15122251