ph2p
(Plasmid
#22520)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonephGf
- Backbone size w/o insert (bp) 3973
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMPyV VP2
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Alt nameMouse Polyomavirus
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Alt nameMurine Polyomavirus
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Alt namecodon modified
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SpeciesMurine Polyomavirus
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Insert Size (bp)959
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GenBank IDFJ548571
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
First reference to this plasmid was in:
Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS. Int J Cancer. 2009 Sep 15.125(6):1250-6
Genbank style annotations can be found at:
http://home.ccr.cancer.gov/LCO/packaging.htm
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ph2p was a gift from Christopher Buck (Addgene plasmid # 22520 ; http://n2t.net/addgene:22520 ; RRID:Addgene_22520) -
For your References section:
Quantitation of human seroresponsiveness to Merkel cell polyomavirus. Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB. PLoS Pathog. 2009 Sep . 5(9):e1000578. 10.1371/journal.ppat.1000578 PubMed 19750217