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Addgene

pGwf
(Plasmid #22517)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22517 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Manufactured in our lab
  • Backbone size (bp) 5874
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdb
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chloramphenicol resistance and CcdB gene
  • Alt name
    CAT
  • Alt name
    control of cell division or death
  • Insert Size (bp)
    1100

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer n/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It differs from phGf in the backbone,
First reference to this plasmid in:
Buck, C.B., et al., Human alpha-defensins block papillomavirus infection. Proc Natl Acad Sci U S A, 2006. 103(5): p. 1516-21.

Genbank style annotations can be found at:
http://home.ccr.cancer.gov/Lco/support.htm

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGwf was a gift from Christopher Buck (Addgene plasmid # 22517 ; http://n2t.net/addgene:22517 ; RRID:Addgene_22517)
  • For your References section:

    Quantitation of human seroresponsiveness to Merkel cell polyomavirus. Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB. PLoS Pathog. 2009 Sep . 5(9):e1000578. 10.1371/journal.ppat.1000578 PubMed 19750217