pGwf
(Plasmid
#22517)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneManufactured in our lab
- Backbone size (bp) 5874
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdb
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChloramphenicol resistance and CcdB gene
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Alt nameCAT
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Alt namecontrol of cell division or death
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Insert Size (bp)1100
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer n/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
It differs from phGf in the backbone,
First reference to this plasmid in:
Buck, C.B., et al., Human alpha-defensins block papillomavirus infection. Proc Natl Acad Sci U S A, 2006. 103(5): p. 1516-21.
Genbank style annotations can be found at:
http://home.ccr.cancer.gov/Lco/support.htm
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGwf was a gift from Christopher Buck (Addgene plasmid # 22517 ; http://n2t.net/addgene:22517 ; RRID:Addgene_22517) -
For your References section:
Quantitation of human seroresponsiveness to Merkel cell polyomavirus. Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB. PLoS Pathog. 2009 Sep . 5(9):e1000578. 10.1371/journal.ppat.1000578 PubMed 19750217