-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 21985 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV4-3HA
-
Backbone manufacturerAndersson,S. et al, J. Biol. Chem. 264 (14), 8222-8229 (1989)
- Backbone size w/o insert (bp) 4874
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameI-kappa B alpha
-
Alt nameIkB-alpha
-
Alt nameNFKBI
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1000
-
Entrez GeneNFKBIA (a.k.a. EDAID2, IKBA, MAD-3, NFKBI)
-
Tag
/ Fusion Protein
- 3x HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Parental vector = pCMV4 3xHA;
3xHA inserted into MluI/HindIII of pCMV4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV4-3 HA/IkB-alpha was a gift from Warner Greene (Addgene plasmid # 21985 ; http://n2t.net/addgene:21985 ; RRID:Addgene_21985) -
For your References section:
Both amino- and carboxyl-terminal sequences within I kappa B alpha regulate its inducible degradation. Sun S, Elwood J, Greene WC. Mol Cell Biol. 1996 Mar . 16(3):1058-65. PubMed 8622650