MBP-3C-hNorrin(33-133)-1D4_pAcGP67a
(Plasmid
#216384)
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PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216384 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcGP67a
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Backbone manufacturerBD Biosciences
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Vector typeInsect Expression ; Baculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNorrin
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Alt nameNDP
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SpeciesH. sapiens (human)
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Insert Size (bp)1600
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Entrez GeneNDP (a.k.a. EVR2, FEVR, ND)
- Promoter Polyhedrin
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Tags
/ Fusion Proteins
- GP64 signal sequence-MBP-3C (N terminal on insert)
- Rho1D4 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.02.03.578714 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MBP-3C-hNorrin(33-133)-1D4_pAcGP67a was a gift from William Weis (Addgene plasmid # 216384 ; http://n2t.net/addgene:216384 ; RRID:Addgene_216384) -
For your References section:
The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling. Bruguera ES, Mahoney JP, Weis WI. bioRxiv 2024.02.03.578714 10.1101/2024.02.03.578714