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Addgene

PM-RA
(Plasmid #211705)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211705 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 4728
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PM-targetted RA
  • Alt name
    Lck N-terminal lipidation signal fused with RA
  • Species
    Synthetic
  • Insert Size (bp)
    765
  • GenBank ID
    NM_001162433.2
  • Entrez Gene
    Lck (a.k.a. Hck-3, Lsk, Lskt, p56<lck>, p56Lck)
  • Promoter CMV
  • Tag / Fusion Protein
    • RA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PM-RA was a gift from Yasunori Saheki (Addgene plasmid # 211705 ; http://n2t.net/addgene:211705 ; RRID:Addgene_211705)
  • For your References section:

    Visualization of accessible cholesterol using a GRAM domain-based biosensor. Koh DHZ, Naito T, Na M, Yeap YJ, Rozario P, Zhong FL, Lim KL, Saheki Y. Nat Commun. 2023 Oct 25;14(1):6773. doi: 10.1038/s41467-023-42498-7. 10.1038/s41467-023-42498-7 PubMed 37880244