PM-RA
(Plasmid
#211705)
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PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Total vector size (bp) 4728
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePM-targetted RA
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Alt nameLck N-terminal lipidation signal fused with RA
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SpeciesSynthetic
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Insert Size (bp)765
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GenBank IDNM_001162433.2
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Entrez GeneLck (a.k.a. Hck-3, Lsk, Lskt, p56<lck>, p56Lck)
- Promoter CMV
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Tag
/ Fusion Protein
- RA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PM-RA was a gift from Yasunori Saheki (Addgene plasmid # 211705 ; http://n2t.net/addgene:211705 ; RRID:Addgene_211705) -
For your References section:
Visualization of accessible cholesterol using a GRAM domain-based biosensor. Koh DHZ, Naito T, Na M, Yeap YJ, Rozario P, Zhong FL, Lim KL, Saheki Y. Nat Commun. 2023 Oct 25;14(1):6773. doi: 10.1038/s41467-023-42498-7. 10.1038/s41467-023-42498-7 PubMed 37880244