pGSTag Flag p38 gamma
(Plasmid
#20818)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGSTag
- Backbone size w/o insert (bp) 5057
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepGSTag Flag p38 gamma
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Alt nameMapK12
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Alt nameSAPK3
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1175
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GenBank IDNM_021746
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Entrez GeneMapk12 (a.k.a. Sapk3)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Xho 1 (not destroyed)
- 5′ sequencing primer pGex 5'
- 3′ sequencing primer pGex 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGSTag Flag p38 gamma was a gift from Roger Davis (Addgene plasmid # 20818 ; http://n2t.net/addgene:20818 ; RRID:Addgene_20818) -
For your References section:
Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen H, Raingeaud J, Davis RJ. J Biol Chem. 1998 Jan 16. 273(3):1741-8. 10.1074/jbc.273.3.1741 PubMed 9430721