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Addgene

pGSTag Flag p38 gamma
(Plasmid #20818)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20818 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGSTag
  • Backbone size w/o insert (bp) 5057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pGSTag Flag p38 gamma
  • Alt name
    MapK12
  • Alt name
    SAPK3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1175
  • GenBank ID
    NM_021746
  • Entrez Gene
    Mapk12 (a.k.a. Sapk3)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xho 1 (not destroyed)
  • 5′ sequencing primer pGex 5'
  • 3′ sequencing primer pGex 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGSTag Flag p38 gamma was a gift from Roger Davis (Addgene plasmid # 20818 ; http://n2t.net/addgene:20818 ; RRID:Addgene_20818)
  • For your References section:

    Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen H, Raingeaud J, Davis RJ. J Biol Chem. 1998 Jan 16. 273(3):1741-8. 10.1074/jbc.273.3.1741 PubMed 9430721