pCMV5 Flag p38 alpha(agf)
(Plasmid
#20787)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
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Backbone manufacturerDr. David Russell, UTSW
- Backbone size w/o insert (bp) 4657
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCMV5 Flag p38 alpha (agf)
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Alt nameMapK14
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SpeciesM. musculus (mouse)
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Insert Size (bp)1127
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Mutationreplaced Thr180 and Tyr182 with Ala and Phe, respectively
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GenBank IDNM_011951
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Entrez GeneMapk14 (a.k.a. CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer CMV F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byoriginal wild-type p38 alpha insert provided by Jiahuai Han and Richard J. Ulevitch, "A Map Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian Cells", (Science,vol.265, pg806)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5 Flag p38 alpha(agf) was a gift from Roger Davis (Addgene plasmid # 20787 ; http://n2t.net/addgene:20787 ; RRID:Addgene_20787) -
For your References section:
Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, Ulevitch RJ, Davis RJ. J Biol Chem. 1995 Mar 31. 270(13):7420-6. 10.1074/jbc.270.13.7420 PubMed 7535770